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验证数据展示

  • WB analysis of HeLa using 86664-3-RR

    WB analysis of HeLa using 86664-3-RR

    WB result of Calpastatin antibody (86664-3-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpastatin transfected HeLa cells.

  • WB analysis using 86664-3-RR

    WB analysis using 86664-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86664-3-RR (CAST antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 86664-3-RR

    IHC staining of human heart using 86664-3-RR

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 86664-3-RR (Calpastatin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human heart using 86664-3-RR

    IHC staining of human heart using 86664-3-RR

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 86664-3-RR (Calpastatin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 86664-3-RR

    IF Staining of HeLa using 86664-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpastatin antibody (86664-3-RR, Clone: 251652G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86664-3-RR

    Affinity and Kinetic Characterization of 86664-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86664-3-RR against Human Calpastatin were performed. The affinity constant is 6.39 nM.

  • KD/KO Validated

Calpastatin Recombinant monoclonal antibody

Calpastatin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86664-3-RR

产品说明书

CloneNo. 251652G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CAST, Calpain inhibitor, Sperm BS-17 component

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MDA-MB-231 cells, A549 cells, HaCaT cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86664-3-RR targets Calpastatin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2897

Product name: Recombinant human CAST protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC013579

Sequence: MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calpastatin
别名 CAST, Calpain inhibitor, Sperm BS-17 component
计算分子量 667 aa, 72 kDa
观测分子量110-130 kDa
GenBank蛋白编号BC013579
基因名称 Calpastatin
Gene ID (NCBI) 831
RRIDAB_3745040
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP20810
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calpastatin (CAST) is an endogenous protein that specifically inhibits the activity of calpains, a family of calcium-dependent proteases involved in numerous cellular processes. The CAST gene encodes calpastatin, which contains multiple calpain-inhibiting domains that bind to the active site of calpain, preventing it from performing its functions. Calpastatin is found in the cytosol and plays a key role in regulating intracellular proteolysis, with malfunctions in the calpain-calpastatin system linked to various diseases, including autoimmune disorders and neurodegenerative conditions.

实验方案

Product Specific Protocols
IF protocol for Calpastatin antibody 86664-3-RRDownload protocol
IHC protocol for Calpastatin antibody 86664-3-RRDownload protocol
WB protocol for Calpastatin antibody 86664-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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