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验证数据展示

  • WB analysis using 55277-1-AP (same clone as 55277-1-PBS)

    WB analysis using 55277-1-AP (same clone as 55277-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 55277-1-AP (same clone as 55277-1-PBS)

    WB analysis of A549 using 55277-1-AP (same clone as 55277-1-PBS)

    WB result of CASK antibody (55277-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CASK transfected A549 cells. This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 55277-1-AP (same clone as 55277-1-PBS)

    IHC staining of mouse kidney using 55277-1-AP (same clone as 55277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASK antibody included
  • IHC staining of human thyroid cancer using 55277-1-AP (same clone as 55277-1-PBS)

    IHC staining of human thyroid cancer using 55277-1-AP (same clone as 55277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASK antibody included
  • IHC staining of rat small intestine using 55277-1-AP (same clone as 55277-1-PBS)

    IHC staining of rat small intestine using 55277-1-AP (same clone as 55277-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CASK antibody included
  • Featured Product
  • KD/KO Validated

CASK Polyclonal antibody, PBS Only

CASK Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 55277-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CASK Ready-To-Use IHC Kit. CASK primary antibody included.

产品信息

55277-1-PBS targets CASK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium/calmodulin-dependent serine protein kinase (MAGUK family)
别名
计算分子量 105 kDa
观测分子量 100-110 kDa, 50-55 kDa
GenBank蛋白编号NM_003688
基因名称 CASK
Gene ID (NCBI) 8573
RRIDAB_10858487
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14936
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CASK, also named as LIN2, belongs to the MAGUK family. It is a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. It contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. CASK binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. It may mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) . Defects in CASK are the cause of FG syndrome type 4 (FGS4). This antibody can recognize all the isoform of CASK.

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