验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells |
| Positive IHC detected in | mouse brain tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15296-1-AP targets CARS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7546 Product name: Recombinant human CARS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC002880 Sequence: MADSSGQQGKGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVFIPQDGKKVTWYCCGPTVYDASHMGHARSYISFDILRRVLKDYFKFDVFYCMNITDIDDKIIKRARQNHLFEQYREKRPEAAQLLEDVQAALKPFSVKLNETTDPDKKQMLERIQHAVQLATEPLEKAVQSRLTGEEVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDNSIFSKLPKFWEGDFHRDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYVSNGSVYFDTAKFASSEKHSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSAMAG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cysteinyl-tRNA synthetase |
| 别名 | CARS1, CYSRS, Cysteine tRNA ligase, Cysteine--tRNA ligase, cytoplasmic, cysteinyl tRNA synthetase |
| 计算分子量 | 89 kDa |
| 观测分子量 | 70-85 kDa |
| GenBank蛋白编号 | BC002880 |
| 基因名称 | CARS |
| Gene ID (NCBI) | 833 |
| RRID | AB_2068455 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49589 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CARS antibody 15296-1-AP | Download protocol |
| IHC protocol for CARS antibody 15296-1-AP | Download protocol |
| WB protocol for CARS antibody 15296-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ann Rheum Dis Mass spectrometry-based identification of new anti-Ly and known antisynthetase autoantibodies | ||
Nitric Oxide H2S alleviated sepsis-induced acute kidney injury by inhibiting PERK/Bax-Bcl2 pathway |
