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CARS Polyclonal antibody

CARS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

Cysteinyl-tRNA synthetase, cysteinyl tRNA synthetase, Cysteine--tRNA ligase, cytoplasmic, Cysteine tRNA ligase, CYSRS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inmouse brain tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15296-1-AP targets CARS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 CARS fusion protein Ag7546 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cysteinyl-tRNA synthetase
别名 Cysteinyl-tRNA synthetase, cysteinyl tRNA synthetase, Cysteine--tRNA ligase, cytoplasmic, Cysteine tRNA ligase, CYSRS
计算分子量 89 kDa
观测分子量 70-85 kDa
GenBank蛋白编号BC002880
基因名称 CARS
Gene ID (NCBI) 833
RRIDAB_2068455
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49589
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CARS antibody 15296-1-APDownload protocol
IHC protocol for CARS antibody 15296-1-APDownload protocol
IF protocol for CARS antibody 15296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

CARS senses cysteine deprivation to activate AMPK for cell survival.

Authors - Mengqiu Yuan
  • KD Validated
humanWB,IP

Ann Rheum Dis

Mass spectrometry-based identification of new anti-Ly and known antisynthetase autoantibodies

Authors - Jean-Baptiste Vulsteke
mouseWB

Nitric Oxide

H2S alleviated sepsis-induced acute kidney injury by inhibiting PERK/Bax-Bcl2 pathway

Authors - Chengqing Song
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