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验证数据展示

  • WB analysis of MCF-7 using 27133-1-AP

    WB analysis of MCF-7 using 27133-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27133-1-AP (CARMIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 27133-1-AP

    IP experiment of MCF-7 using 27133-1-AP

    IP result of anti-CARMIL (IP:27133-1-AP, 4ug; Detection:27133-1-AP 1:1000) with MCF-7 cells lysate 2800 ug.

  • IHC staining of human colon using 27133-1-AP

    IHC staining of human colon using 27133-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27133-1-AP (CARMIL antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 27133-1-AP

    IHC staining of human colon using 27133-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27133-1-AP (CARMIL antibody at dilution of 1:200 (under 40x lens).

CARMIL Polyclonal antibody

CARMIL Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27133-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

CARMIL, CARMIL homolog, CARMIL1a, LRRC16, LRRC16A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HepG2 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

27133-1-AP targets CARMIL in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag25876

Product name: Recombinant human LRRC16A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1021-1365 aa of BC140770

Sequence: RVDEGVDEFFTKKVTKMDSKKWSTRGSESHELNEGGDEKKKRDSRKSSGFLNLIKSRSKSERPPTILMTEEPSSPKGAVRSPPVDCPRKDTKAAEHNGNSERIEEIKTPDSFEESQGEEIGKVERSDSKSSPQAGRRYGVQVMGSGLLAEMKAKQEKRAACAQKKLGNDAVSQDSSSPALSSVERSDGGGAGLPENRFGLGTPEKNTKAEPKAEAGSRSRSSSSTPTSPKPLLQSPKPSLAARPVIPQKPRTASRPDDIPDSPSSPKVALLPPVLKKVPSDKERDGQSSPQPSPRTFSQEVSRRSWGQQAQEYQEQKQRSSSKDGHQGSKSNDSGEEAEKEFIFV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine rich repeat containing 16A
别名 CARMIL, CARMIL homolog, CARMIL1a, LRRC16, LRRC16A
观测分子量 152 kDa
GenBank蛋白编号BC140770
基因名称 CARMIL1
Gene ID (NCBI) 55604
RRIDAB_2880768
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VZK9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CARMIL antibody 27133-1-APDownload protocol
IHC protocol for CARMIL antibody 27133-1-APDownload protocol
IP protocol for CARMIL antibody 27133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Int Immunopharmacol

CARMIL1 regulates liver cancer cell proliferation by activating the ERK/mTOR pathway through the TRIM27/p53 axis

Authors - Yuzhen Ge
View Article