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CARMIL Polyclonal antibody

CARMIL Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 27133-1-AP

Print datasheet

Synonyms

CARMIL, CARMIL homolog, CARMIL1a, LRRC16, LRRC16A

验证数据展示

  • WB analysis of MCF-7 using 27133-1-AP

    WB analysis of MCF-7 using 27133-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27133-1-AP (CARMIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 27133-1-AP

    IP experiment of MCF-7 using 27133-1-AP

    IP result of anti-CARMIL (IP:27133-1-AP, 4ug; Detection:27133-1-AP 1:1000) with MCF-7 cells lysate 2800 ug.

  • IHC staining of human colon using 27133-1-AP

    IHC staining of human colon using 27133-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27133-1-AP (CARMIL antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 27133-1-AP

    IHC staining of human colon using 27133-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27133-1-AP (CARMIL antibody at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inMCF-7 cells, HepG2 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27133-1-AP is CARMIL Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human
Immunogen CARMIL fusion protein Ag25876 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine rich repeat containing 16A
Synonyms CARMIL, CARMIL homolog, CARMIL1a, LRRC16, LRRC16A
Observed Molecular Weight 152 kDa
GenBank Accession NumberBC140770
Gene Symbol LRRC16A
Gene ID (NCBI) 55604
RRIDAB_2880768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VZK9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CARMIL antibody 27133-1-APDownload protocol
IHC protocol for CARMIL antibody 27133-1-APDownload protocol
IP protocol for CARMIL antibody 27133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols