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验证数据展示

  • IHC staining of mouse testis using 83300-2-RR (same clone as 83300-2-PBS)

    IHC staining of mouse testis using 83300-2-RR (same clone as 83300-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83300-2-RR (CARHSP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83300-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83300-2-RR (same clone as 83300-2-PBS)

    IF Staining of HepG2 using 83300-2-RR (same clone as 83300-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CARHSP1 antibody (83300-2-RR, Clone: 230489F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83300-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83300-2-RR (same clone as 83300-2-PBS)

    FC experiment of HepG2 using 83300-2-RR (same clone as 83300-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CARHSP1 Recombinant antibody (83300-2-RR, Clone:230489F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83300-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83300-2-RR

    Affinity and Kinetic Characterization of 83300-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83300-2-RR against Human CARHSP1 were performed. The affinity constant is 4.36 nM.

CARHSP1 Recombinant antibody, PBS Only

CARHSP1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83300-2-PBS

产品说明书

CloneNo. 230489F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83300-2-PBS targets CARHSP1 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 CARHSP1 fusion protein Ag2265 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium regulated heat stable protein 1, 24kDa
别名 CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1
计算分子量 147 aa, 16 kDa
GenBank蛋白编号BC003366
基因名称 CARHSP1
Gene ID (NCBI) 23589
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y2V2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C