验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse liver tissue, mouse testis tissue, rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11672-1-AP targets CARHSP1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2265 Product name: Recombinant human CARHSP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC003366 Sequence: MSSEPPPPPQPPTHQASVGLLDTPRSRERSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHISDVEGEYVPVEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGHVISS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calcium regulated heat stable protein 1, 24kDa |
| 别名 | Calcium-regulated heat-stable protein 1, Calcium-regulated heat-stable protein of 24 kDa, CRHSP 24, CRHSP-24, CSDC1 |
| 计算分子量 | 147 aa, 16 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC003366 |
| 基因名称 | CARHSP1 |
| Gene ID (NCBI) | 23589 |
| RRID | AB_3669130 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2V2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CARHSP1(calcium-regulated heat-stable protein 1, a 24 kDa protein also known as CRHSP-24) is a ubiquitously expressed phosphoprotein that is comprised of 147 amino acids with nearly 14% proline (PMID: 21990353,30257675). CARHSP1 was discovered as a biomarker for diabetic retinopathy and as a regulator of TNF-α mRNA stability in macrophages (PMID: 21078874). CARHSP1 may be involved in oxidative stress via traffic between stress granules and processing bodies (PMID: 21177848).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CARHSP1 antibody 11672-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
