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CARD8 Polyclonal antibody

CARD8 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14516-1-AP

Print datasheet

Synonyms

Apoptotic protein NDPP1, CARD8, CARDINAL, DACAR, Dakar, KIAA0955, NDPP, NDPP1, TUCAN

验证数据展示

  • WB analysis using 14516-1-AP

    WB analysis using 14516-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14516-1-AP (CARD8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 14516-1-AP

    IF Staining of HepG2 using 14516-1-AP

    Immunofluorescent analysis of HepG2 cells, using CARD8 antibody 14516-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human prostate cancer using 14516-1-AP

    IHC staining of human prostate cancer using 14516-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 14516-1-AP

    IHC staining of human prostate cancer using 14516-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HUVEC cells, THP-1 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14516-1-AP targets CARD8 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human
Immunogen CARD8 fusion protein Ag5975 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caspase recruitment domain family, member 8
Synonyms Apoptotic protein NDPP1, CARD8, CARDINAL, DACAR, Dakar, KIAA0955, NDPP, NDPP1, TUCAN
Calculated Molecular Weight61 kDa, 49 kDa
Observed Molecular Weight61 kDa, 49 kDa
GenBank Accession NumberBC056891
Gene Symbol CARD8
Gene ID (NCBI) 22900
RRIDAB_2068262
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2G2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CARD8 belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). CARD8 may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis.

实验方案

Product Specific Protocols
WB protocol for CARD8 antibody 14516-1-APDownload protocol
IHC protocol for CARD8 antibody 14516-1-APDownload protocol
IF protocol for CARD8 antibody 14516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols