验证数据展示
经过测试的应用
| Positive WB detected in | EC109 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28373-1-AP targets CARD14 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | CARD14 fusion protein Ag28969 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | caspase recruitment domain family, member 14 |
| 别名 | BIMP2, CARD14, Carma 2, CARMA2 |
| 计算分子量 | 113 kDa |
| 观测分子量 | 110-140 kDa |
| GenBank蛋白编号 | BC018142 |
| 基因名称 | CARD14 |
| Gene ID (NCBI) | 79092 |
| RRID | AB_3669656 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BXL6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CARD14, also known as CARMA2, is closely related to CARD9, CARD10 , and CARD11 , which upon activation, each form an oligomeric CBM signaling complex with B‐cell lymphoma 10 (BCL10) and mucosa‐associated lymphoid tissue lymphoma translocation protein 1 (MALT1). It is predominantly expressed in epithelial cells of esophagus, skin, and mucosal tissues, although some isoforms were also shown to be expressed in hematopoietic cell lines.(PMID: 36009554, PMID: 32343482)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CARD14 antibody 28373-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |