验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17103-1-AP targets CAPZA3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag10774 Product name: Recombinant human CAPZA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-299 aa of BC016745 Sequence: MTLSVLSRKDKERVIRRLLLQAPPGEFVNAFDDLCLLIRDEKLMHHQGECAGHQHCQKYSVPLCIDGNPVLLSHHNVMGDYRFFDHQSKLSFKYYLLQNQLKDIQSHGIIQNEAEYLRVVLLCALKLYVNDHYPKGNCNMLRKTVKSKEYLIACIEDHNYETGECWNGLWKSKWIFQVNPFLTQVTGRIFVQAHFFRCVNLHIEISKDLKESLEIVNQAQLALSFARLVEEQENKFQAAVLEELQELSNEALRKILRRDLPVTRTLIDWHRILSDLNLVMYPKLGYVIYSRSVLCNWII 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | capping protein (actin filament) muscle Z-line, alpha 3 |
| 别名 | CAPAA3, CAPPA3, CapZ alpha-3, CP-alpha-3, F-actin-capping protein subunit alpha-3 |
| 计算分子量 | 299 aa, 35 kDa |
| 观测分子量 | 30-35 kDa |
| GenBank蛋白编号 | BC016745 |
| 基因名称 | CAPZA3 |
| Gene ID (NCBI) | 93661 |
| RRID | AB_3741905 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96KX2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAPZA3 encodes an actin capping protein, one of the F-actin capping protein alpha subunit family. In vertebrates, the α subunit is encoded by three CAPZA genes, CAPZA1, CAPZA2 and CAPZA3, which encode α1, α2 and α3 subunits, respectively(PMID: 32338762). CAPZA3 is expressed exclusively in the testis and sperm(PMID: 12029070).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CAPZA3 antibody 17103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
