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验证数据展示

  • WB analysis of mouse skin using 15803-1-AP

    WB analysis of mouse skin using 15803-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 15803-1-AP (Calpain S2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skin using 15803-1-AP

    IP experiment of mouse skin using 15803-1-AP

    IP result of anti-Calpain S2 (IP:15803-1-AP, 4ug; Detection:15803-1-AP 1:300) with mouse skin tissue lysate 2000ug.

  • IHC staining of human skin cancer using 15803-1-AP

    IHC staining of human skin cancer using 15803-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15803-1-AP (Calpain S2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 15803-1-AP

    IHC staining of human skin cancer using 15803-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15803-1-AP (Calpain S2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Calpain S2 Polyclonal antibody

Calpain S2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15803-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

CAPNS2, CSS2, calpain, small subunit 2, Calpain small subunit 2, Calcium-dependent protease small subunit 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skin tissue, rat skin tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15803-1-AP targets Calpain S2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8534

Product name: Recombinant human CAPNS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 5-248 aa of BC005397

Sequence: KALLEGADRGLGEALGGLFGGGGQRREGGGRNIGGIVGGIVNFISEAAAAQYTPEPPPTQQHFTSVEASESEEVRRFRQQFTQLAGPDMEVGATDLMNILNKVLSKHKDLKTDGFSLDTCRSIVSVMDSDTTGKLGFEEFKYLWNNIKKWQCVYKQYDRDHSGSLGSSQLRGALQAAGFQLNEQLYQMIVRRYANEDGDMDFNNFISCLVRLDAMFRAFKSLDRDRDGLIQVSIKEWLQLTMYS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calpain, small subunit 2
别名 CAPNS2, CSS2, calpain, small subunit 2, Calpain small subunit 2, Calcium-dependent protease small subunit 2
计算分子量 248 aa, 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC005397
基因名称 Calpain S2
Gene ID (NCBI) 84290
RRIDAB_2259580
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96L46
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calpain S2, also known as calpain-2, is a member of the calpain family of calcium-dependent proteases that play crucial roles in various cellular processes.

实验方案

Product Specific Protocols
WB protocol for Calpain S2 antibody 15803-1-APDownload protocol
IHC protocol for Calpain S2 antibody 15803-1-APDownload protocol
IP protocol for Calpain S2 antibody 15803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols