验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, A431 cells, HeLa cells, K-562 cells, THP-1 cells, HSC-T6 cells, PC-12 cells |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy CAPNS1 Ready-To-Use IHC Kit. CAPNS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67838-1-Ig targets CAPNS1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag21885 Product name: Recombinant human CAPNS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-323 aa of BC011903 Sequence: MFLVNSFLKGGGGGGGGGGGLGGGLGNVLGGLISGAGGGGGGGGGGGGGGGGGGGGTAMRILGGVISAISEAAAQYNPEPPPPRTHYSNIEANESEEVRQFRRLFAQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDSDTTGKLGFEEFKYLWNNIKRWQAIYKQFDTDRSGTICSSELPGAFEAAGFHLNEHLYNMIIRRYSDESGNMDFDNFISCLVRLDAMFRAFKSLDKDGTGQIQVNIQEVRTPILGYGCLGGPHPSALHTSSELQSPSSYFASRPWVRAKGLVLLGFPVLTLHPPLPSGCS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | calpain, small subunit 1 |
| 别名 | CAPN4, CAPNS, 2B6G6, Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit |
| 计算分子量 | 28 kDa |
| GenBank蛋白编号 | BC011903 |
| 基因名称 | CAPNS1 |
| Gene ID (NCBI) | 826 |
| RRID | AB_2918600 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P04632 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CAPNS1 antibody 67838-1-Ig | Download protocol |
| WB protocol for CAPNS1 antibody 67838-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
