验证数据展示
经过测试的应用
| Positive IHC detected in | mouse stomach tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20971-1-AP targets CAPN8 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calpain 8 |
| 别名 | calpain 8, nCL 2, NCL2, New calpain 2 |
| 计算分子量 | 79 kDa |
| GenBank蛋白编号 | NM_001143962 |
| 基因名称 | CAPN8 |
| Gene ID (NCBI) | 388743 |
| RRID | AB_3085628 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A6NHC0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAPN8 (Calpain-8) was found to be a significant risk factor for THCA (Thyroid cancer) with a markedly elevated level of mRNA and protein in tumor tissues. This potential oncogene could induce the activation of epithelial mesenchymal transition and E2F-targeted pathways (PMID:36389799).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CAPN8 antibody 20971-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
