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验证数据展示

  • WB analysis of human heart using 13870-1-AP

    WB analysis of human heart using 13870-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13870-1-AP (Calpain 7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 13870-1-AP

    IHC staining of human ovary cancer using 13870-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13870-1-AP (Calpain 7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 13870-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 13870-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13870-1-AP (Calpain 7 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Calpain 7 Polyclonal antibody

Calpain 7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13870-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CAPN7, PalBH, EC:3.4.22.-, Calpain-7, CALPAIN7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13870-1-AP targets Calpain 7 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4820

Product name: Recombinant human CAPN7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 235-533 aa of BC056202

Sequence: FAYPMPFCDRWGKLPLSPKQKTTFSKWVRPEDLTNNPTMIYTVSSFSIKQTIVSDCSFVASLAISAAYERRFNKKLITGIIYPQNKDGEPEYNPCGKYMVKLHLNGVPRKVIIDDQLPVDHKGELLCSYSNNKSELWVSLIEKAYMKVMGGYDFPGSNSNIDLHALTGWIPERIAMHSDSQTFSKDNSFRMLYQRFHKGDVLITASTGMMTEAEGEKWGLVPTHAYAVLDIREFKGLRFIQLKNPWSHLRWKGRYSENDVENWTPELQKYLNFDPRTAQKIDNGIFWISWDDLCQYYDV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calpain 7
别名 CAPN7, PalBH, EC:3.4.22.-, Calpain-7, CALPAIN7
计算分子量 93 kDa
观测分子量 92 kDa
GenBank蛋白编号BC056202
基因名称 Calpain 7
Gene ID (NCBI) 23473
RRIDAB_2069823
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6W3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Calpain 7 antibody 13870-1-APDownload protocol
WB protocol for Calpain 7 antibody 13870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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