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验证数据展示

  • WB analysis using 32282-1-AP

    WB analysis using 32282-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32282-1-AP (CAND1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 32282-1-AP

    IP experiment of HEK-293 using 32282-1-AP

    IP result of anti-CAND1 (IP:32282-1-AP, 4ug; Detection:32282-1-AP 1:2000) with HEK-293 cells lysate 1280 ug.

CAND1 Polyclonal antibody

CAND1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 32282-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

Cullin-associated and neddylation-dissociated protein 1, Cullin-associated NEDD8-dissociated protein 1, KIAA0829, p120 CAND1, TBP-interacting protein 120A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, MDA-MB-231 cells
Positive IP detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32282-1-AP targets CAND1 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35297

Product name: Recombinant human CAND1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 396-586 aa of NM_018448

Sequence: HAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cullin-associated and neddylation-dissociated 1
别名 Cullin-associated and neddylation-dissociated protein 1, Cullin-associated NEDD8-dissociated protein 1, KIAA0829, p120 CAND1, TBP-interacting protein 120A
计算分子量136 kDa
观测分子量120 kDa
GenBank蛋白编号NM_018448
基因名称 CAND1
Gene ID (NCBI) 55832
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86VP6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAND1 (Cullin-Associated and Neddylation-Dissociated 1) is a key regulatory protein involved in the proper functioning of the CRL (Cullin-RING ubiquitin ligase) family, which plays a crucial role in ubiquitin-mediated protein degradation (PMID: 18444905).

实验方案

Product Specific Protocols
IP protocol for CAND1 antibody 32282-1-APDownload protocol
WB protocol for CAND1 antibody 32282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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