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验证数据展示

  • WB analysis using 83864-1-RR

    WB analysis using 83864-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83864-1-RR (CAMSAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of Neuro-2a using 83864-1-RR

    IF Staining of Neuro-2a using 83864-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CAMSAP1 antibody (83864-1-RR, Clone: 240633H1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CAMSAP1 Recombinant antibody

CAMSAP1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83864-1-RR

产品说明书

CloneNo. 240633H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

PRO2405, Calmodulin-regulated spectrin-associated protein 1, 240633H1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, Neuro-2a cells
Positive IF/ICC detected inNeuro-2a cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83864-1-RR targets CAMSAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calmodulin regulated spectrin-associated protein 1
别名 PRO2405, Calmodulin-regulated spectrin-associated protein 1, 240633H1
计算分子量 179 kDa
观测分子量150-160 kDa
GenBank蛋白编号BC144082
基因名称 CAMSAP1
Gene ID (NCBI) 157922
RRIDAB_3671450
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ5T5Y3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are three members in the CAMSAP family of mammals, namely CAMSAP1-3. Despite having similar structures, these proteins have different distribution patterns within intracellular spaces and tissues. CAMSAP1 (Calmodulin-regulated spectrin-associated protein 1) is mainly expressed in nervous tissue, testis, and liver (PMID: 37903275).

实验方案

Product Specific Protocols
WB protocol for CAMSAP1 antibody 83864-1-RRDownload protocol
IF protocol for CAMSAP1 antibody 83864-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols