验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27970-1-AP targets CAMKMT in WB, IF/ICC, ELISA applications and shows reactivity with Mouse, Rat, Human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | Mouse, Rat, Human |
| 免疫原 |
CatNo: Ag27559 Product name: Recombinant human C2orf34 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 168-311 aa of BC053733 Sequence: SADVKEVLLTDGNEKAIRNVQDIITRNQKAGVFKTQKISSCVLRWDNETDVSQLEGHFDIVMCADCLFLDQYRASLVDAIKRLLQPRGKAMVFAPRRGNTLNQFCNLAEKAGFCIQRHENYDEHISNFHSKLKKENPDIYEENL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 2 open reading frame 34 |
| 别名 | C2orf34, CaM KMT, CAMKMT, CLNMT, FLJ23451 |
| 计算分子量 | 323 aa, 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC053733 |
| 基因名称 | CAMKMT |
| Gene ID (NCBI) | 79823 |
| RRID | AB_3669631 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z624 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAMKMT, also named as C2orf34 and CLNMT, belongs to the class I-like SAM-binding methyltransferase superfamily and CLNMT methyltransferase family. The CAMKMT enzyme participates in cell cycle and development, regulating the production of calmodulin with three methyl lysine residues directly or indirectly. It also plays a major role in the calcium-dependent signaling pathway and posttranscriptional modifications, including Lys-115. CAMKMT is expressed mainly in the brain, liver, muscle, colon, and lung and is involved in muscle cell activities that are regulated by calcium binding (PMID: 27435482). The molecular weight of CAMKMT is 36 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CAMKMT antibody 27970-1-AP | Download protocol |
| WB protocol for CAMKMT antibody 27970-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
