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验证数据展示

  • WB analysis of Jurkat using 11549-1-AP (same clone as 11549-1-PBS)

    WB analysis of Jurkat using 11549-1-AP (same clone as 11549-1-PBS)

    WB result of CAMKK2 antibody (11549-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAMKK2 transfected Jurkat cells. This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

  • WB analysis using 11549-1-AP (same clone as 11549-1-PBS)

    WB analysis using 11549-1-AP (same clone as 11549-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

  • IP experiment of PC-3 using 11549-1-AP (same clone as 11549-1-PBS)

    IP experiment of PC-3 using 11549-1-AP (same clone as 11549-1-PBS)

    IP result of anti-CAMKK2 (IP:11549-1-AP, 4ug; Detection:11549-1-AP 1:500) with PC-3 cells lysate 960 ug. This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 11549-1-AP (same clone as 11549-1-PBS)

    IHC staining of human prostate cancer using 11549-1-AP (same clone as 11549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11549-1-AP (CAMKK2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAMKK2 antibody included
  • IHC staining of human prostate cancer using 11549-1-AP (same clone as 11549-1-PBS)

    IHC staining of human prostate cancer using 11549-1-AP (same clone as 11549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11549-1-AP (CAMKK2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAMKK2 antibody included
  • IF Staining of PC-3 using 11549-1-AP (same clone as 11549-1-PBS)

    IF Staining of PC-3 using 11549-1-AP (same clone as 11549-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CAMKK2 antibody (11549-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11549-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CAMKK2 Polyclonal antibody, PBS Only

CAMKK2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11549-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CAMKK2 Ready-To-Use IHC Kit. CAMKK2 primary antibody included.

产品信息

11549-1-PBS targets CAMKK2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2093

Product name: Recombinant human CAMKK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-353 aa of BC026060

Sequence: MSSCVSSQPSSNRAAPQDELGGRGSSSSESQKPCEALRGLSSLSIHLGMESFIVVTECEPGCAVDLGLARDRPLEADGQEVPLDSSGSQARPHLSGRKLSLQERSQGGLAAGGSLDMNGRCICPSLPYSPVSSPQSSPRLPRRPTVESHHVSITGMQDCVQLNQYTLKDEIGKGSYGVVKLAYNENDNTYYAMKVLSKKKLIRQAGFPRRPPPRGTRPAPGGCIQPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDDPNEDHLYMVFELVNQGPVMEVPTLKPLSEDQARFYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDGHIKIADFGVSNEFKGSDALLSNTVGTPAF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase kinase 2, beta
别名
计算分子量 588 aa, 60 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC026060
基因名称 CAMKK2
Gene ID (NCBI) 10645
RRIDAB_2259441
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RR4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CAMKK2(calcium/calmodulin-dependent protein kinase kinase 2) is also named as CAMKKB, KIAA0787 and belongs to the protein kinase superfamily. It regulates hypothalamic production of the orexigenic hormone NPY and contributes to the regulation of energy balance. CAMKK2 in vivo reduces tumor growth suggests that reactivation of CAMKK2 by development of new drugs may be a new strategy to prolong the period to respond androgen-deprivation therapy(ADT)(PMID:22549914). It can be autophosphorylated(PMID:19369195). It has 7 isoforms produced by alternative splicing.

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