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验证数据展示

  • WB analysis using 68519-1-Ig

    WB analysis using 68519-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68519-1-Ig (CAMK2G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68519-1-Ig

    WB analysis using 68519-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68519-1-Ig (CAMK2G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CAMK2G Monoclonal antibody

CAMK2G Monoclonal Antibody for WB, ELISA
Cat No. 68519-1-Ig

产品说明书

CloneNo. 1C12H10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, ELISA

别名

1C12H10, Calcium/calmodulin-dependent protein kinase type II subunit gamma, CaM kinase II subunit gamma, CAMK, CAMK-II

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, pig brain tissue, LNCaP cells, HCT 116 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy CAMK2G Ready-To-Use IHC Kit. CAMK2G primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68519-1-Ig targets CAMK2G in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag23180

Product name: Recombinant human CAMK2G protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 218-527 aa of BC034044

Sequence: DQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITADQALKHPWVCQRSTVASMMHRQETVECLRKFNARRKLKGAILTTMLVSRNFSVGRQSSAPASPAASAAGLAGQAAKSLLNKKSDGGVKKRKSSSSVHLMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVEGMDFHKFYFENLLSKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWLNVHYHCSGAPAAPLQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase II gamma
别名 1C12H10, Calcium/calmodulin-dependent protein kinase type II subunit gamma, CaM kinase II subunit gamma, CAMK, CAMK-II
计算分子量 527 aa, 59 kDa
观测分子量64 kDa
GenBank蛋白编号BC034044
基因名称 CAMK2G
Gene ID (NCBI) 818
RRIDAB_3085228
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ13555
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G is expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology.

实验方案

Product Specific Protocols
WB protocol for CAMK2G antibody 68519-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols