• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CaMKII Gamma Polyclonal antibody

CaMKII Gamma Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12666-2-AP

Print datasheet

Synonyms

CaM kinase II subunit gamma, CAMK, CAMK II, CaMK II subunit gamma, CAMK2G, CAMKG, CaMKII Gamma

验证数据展示

  • IHC staining of human pancreas cancer using 12666-2-AP

    IHC staining of human pancreas cancer using 12666-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12666-2-AP (CaMKII gamma antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of HepG2 using 12666-2-AP

    IF Staining of HepG2 using 12666-2-AP

    Immunofluorescent analysis of HepG2 cells, using CAMK2G antibody 12666-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • WB analysis of SMMC-7721 using 12666-2-AP

    WB analysis of SMMC-7721 using 12666-2-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse heart using 12666-2-AP

    IP experiment of mouse heart using 12666-2-AP

    IP result of anti-CaMKII Gamma (IP:12666-2-AP, 4ug; Detection:12666-2-AP 1:1000) with mouse heart tissue lysate 3200ug.

经过测试的应用

Positive WB detected inSMMC-7721 cells, human brain tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12666-2-AP targets CaMKII Gamma in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen CaMKII Gamma fusion protein Ag3348 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium/calmodulin-dependent protein kinase II gamma
Synonyms CaM kinase II subunit gamma, CAMK, CAMK II, CaMK II subunit gamma, CAMK2G, CAMKG, CaMKII Gamma
Calculated Molecular Weight 527 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC034044
Gene Symbol CAMK2G
Gene ID (NCBI) 818
RRIDAB_2068213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13555
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G has been shown to be expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology.

实验方案

Product Specific Protocols
WB protocol for CaMKII Gamma antibody 12666-2-APDownload protocol
IHC protocol for CaMKII Gamma antibody 12666-2-APDownload protocol
IF protocol for CaMKII Gamma antibody 12666-2-APDownload protocol
IP protocol for CaMKII Gamma antibody 12666-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Neuron

JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.

Authors - Jamee M Berg
View Article
mouseWB

Transl Res

Cardiac-Specific Overexpression of CREM-IbΔC-X via CRISPR/Cas9 in Mice Presents a New Model of Atrial Cardiomyopathy with Spontaneous Atrial Fibrillation

Authors - Jiuzhou Chen
View Article
humanWB

Oncotarget

PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.

Authors - Chao Li
View Article
mouseIF

Development

Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse.

Authors - Chang Heng-Yu HY
View Article
humanWB,IF

Aging (Albany NY)

CAMKIIγ is a targetable driver of multiple myeloma through CaMKIIγ/ Stat3 axis.

Authors - Linlin Yang
  • KD Validated
View Article
humanWB

Cell Commun Signal

Wnt5a-induced M2 polarization of tumor-associated macrophages via IL-10 promotes colorectal cancer progression.

Authors - Qing Liu
View Article