验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, HeLa cells, mouse thymus tissue, rat pancreas tissue |
| Positive IHC detected in | mouse brain tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13613-1-AP targets CAMK1D in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4517 Product name: Recombinant human CAMK1D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-198 aa of BC035745 Sequence: ENGESSSSWKKQAEDIKKIFEFKETLGTGAFSEVVLAEEKATGKLFAVKCIPKKALKGKESSIENEIAVLRKIKHENIVALEDIYESPNHLYLVMQLVSGGELFDRIVEKGFYTEKDASTLIRQVLDAVYYLHRMGIVHRDLKPENLLYYSQDEESKIMISDFGLSKMEGKGDVMSTACGTPGYVAPEVLAQKPY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calcium/calmodulin-dependent protein kinase ID |
| 别名 | Calcium/calmodulin-dependent protein kinase type 1D, CaM K1, CaM KI delta, CaM kinase I delta, CaM kinase ID |
| 计算分子量 | 357 aa, 40 kDa |
| 观测分子量 | 38-42 kDa |
| GenBank蛋白编号 | BC035745 |
| 基因名称 | CAMK1D |
| Gene ID (NCBI) | 57118 |
| RRID | AB_2228498 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IU85 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAMK1D (calcium/calmodulin-dependent protein kinase ID) is a serine/threonine kinase belonging to the calcium/calmodulin-dependent protein kinase 1 family. It functions as a component of a calcium-triggered signaling cascade, playing roles in regulating granulocyte function, activating CREB-dependent gene transcription, and influencing neutrophil chemotaxis and apoptosis. CAMK1D is involved in diverse biological processes including nervous system development and inflammatory responses.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CAMK1D antibody 13613-1-AP | Download protocol |
| WB protocol for CAMK1D antibody 13613-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
