验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, human placenta tissue, K-562 cells, mouse kidney tissue |
| Positive IHC detected in | human placenta tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CALU Ready-To-Use IHC Kit. CALU primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17804-1-AP targets Calumenin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, pig |
| 免疫原 |
CatNo: Ag12073 Product name: Recombinant human CALU protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-315 aa of BC013383 Sequence: MDLRQFLMCLSLCTAFALSKPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calumenin |
| 别名 | CALU, Calumenin, Crocalbin, IEF SSP 9302 |
| 计算分子量 | 315 aa, 37 kDa |
| 观测分子量 | 47 kDa, 57 kDa |
| GenBank蛋白编号 | BC013383 |
| 基因名称 | Calumenin |
| Gene ID (NCBI) | 813 |
| RRID | AB_2069964 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43852 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Calumenin antibody 17804-1-AP | Download protocol |
| IHC protocol for Calumenin antibody 17804-1-AP | Download protocol |
| IF protocol for Calumenin antibody 17804-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Oncogene Extracellular calumenin suppresses ERK1/2 signaling and cell migration by protecting fibulin-1 from MMP-13-mediated proteolysis.
| ||
Cancer Med Five EMT-related genes signature predicts overall survival and immune environment in microsatellite instability-high gastric cancer. | ||
J Inflamm Res Pan-Cancer Screening and Validation of CALU's Role in EMT Regulation and Tumor Microenvironment in Triple-Negative Breast Cancer | ||
Respir Res CALU promotes lung adenocarcinoma progression by enhancing cell proliferation, migration and invasion |
