验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, human brain tissue, mouse brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11477-1-AP targets CALN1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 |
CatNo: Ag2043 Product name: Recombinant human CALN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-219 aa of BC020200 Sequence: MPFHHVTAGLLYKGNYLNRSLSAGSDSEQLANISVEELDEIREAFRVLDRDGNGFISKQELGMAMRSLGYMPSEVELAIIMQRLDMDGDGQVDFDEFMTILGPKLVSSEGRDGFLGNTIDSIFWQFDMQRITLEELKHILYHAFRDHLTMKDIENIIINEEESLNETSGNCQTEFEGVHSQKQNRQTCVRKSLICAFAMAFIISVMLIAANQILRSGME 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calneuron 1 |
| 别名 | CaBP8, Calcium binding protein 8, CALN1, calneuron 1, Calneuron I |
| 计算分子量 | 219 aa, 25 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC020200 |
| 基因名称 | CALN1 |
| Gene ID (NCBI) | 83698 |
| RRID | AB_2228454 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BXU9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CALN1 antibody 11477-1-AP | Download protocol |
| IP protocol for CALN1 antibody 11477-1-AP | Download protocol |
| WB protocol for CALN1 antibody 11477-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids circEYA1 Functions as a Sponge of miR-582-3p to Suppress Cervical Adenocarcinoma Tumorigenesis via Upregulating CXCL14. | ||
Hypertension Calneuron 1 Increased Ca2+ in the Endoplasmic Reticulum and Aldosterone Production in Aldosterone-Producing Adenoma. | ||
J Biol Chem Post-translational membrane insertion of tail-anchored transmembrane EF-hand Ca2+ sensor calneurons requires the TRC40/Asna1 protein chaperone. | ||
J Cell Mol Med Identification of core genes in prefrontal cortex and hippocampus of Alzheimer's disease based on mRNA-miRNA network | ||
Biochem Biophys Res Commun Exosomal miR-675 from metastatic osteosarcoma promotes cell migration and invasion by targeting CALN1. |
