验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse thymus tissue, human colon tissue, rat kidney tissue |
| Positive IP detected in | mouse thymus tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15894-1-AP targets CALML4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag8705 Product name: Recombinant human CALML4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 49-196 aa of BC009516 Sequence: QPRPQNEYKECFSLYDKQQRGKIKATDLMVAMRCLGASPTPGEVQRHLQTHGIDGNGELDFSTFLTIMHMQIKQEDPKKEILLAMLMVDKEKKGYVMASDLRSKLTSLGEKLTHKEVDDLFREADIEPNGKVKYDEFIHKITLPGRDY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calmodulin-like 4 |
| 别名 | CALML4, calmodulin like 4, Calmodulin like protein 4, NY BR 20 |
| 计算分子量 | 196 aa, 22 kDa |
| 观测分子量 | 22-25 kDa |
| GenBank蛋白编号 | BC009516 |
| 基因名称 | CALML4 |
| Gene ID (NCBI) | 91860 |
| RRID | AB_2069462 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96GE6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for CALML4 antibody 15894-1-AP | Download protocol |
| WB protocol for CALML4 antibody 15894-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Chem Neurosci Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine. | ||
J Biol Chem The small EF-hand protein CALML4 functions as a critical myosin light chain within the intermicrovillar adhesion complex.
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