验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, RAW264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23136-1-AP targets CADM4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag18922 Product name: Recombinant human CADM4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-321 aa of BC141627 Sequence: AGQEVQTENVTVAEGGVAEITCRLHQYDGSIVVIQNPARQTLFFNGTRALKDERFQLEEFSPRRVRIRLSDARLEDEGGYFCQLYTEDTHHQIATLTVLVAPENPVVEVREQAVEGGEVELSCLVPRSRPAATLRWYRDRKELKGVSSSQENGKVWSVASTVRFRVDRKDDGGIIICEAQNQALPSGHSKQTQYVLDVQYSPTARIHASQAVVREGDTLVLTCAVTGNPRPNQIRWNRGNESLPERAEAVGETLTLPGLVSADNGTYTCEASNKHGHARALYVLVVYDPGAVVEAQTSV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cell adhesion molecule 4 |
| 别名 | CADM4, cell adhesion molecule 4, IGSF4C, Necl 4, NECL4, Nectin like protein 4, synCAM4, TSLC1 like protein 2, TSLL2 |
| 计算分子量 | 388 aa, 43 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC141627 |
| 基因名称 | CADM4 |
| Gene ID (NCBI) | 199731 |
| RRID | AB_2879215 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NFZ8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CADM4, also known as IGSF4C or TSLL2, is a cell adhesion molecule belonging to the immunoglobulin superfamily. CADM4 has been shown to serve as a tumor suppressor.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CADM4 antibody 23136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
