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CADM3 Polyclonal antibody

CADM3 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15660-1-AP

Print datasheet

Synonyms

BIgR, Brain immunoglobulin receptor, CADM3, cell adhesion molecule 3, IGSF4B, Necl 1, NECL1, Nectin like protein 1, synCAM3, TSLC1 like protein 1, TSLL1

验证数据展示

  • WB analysis using 15660-1-AP

    WB analysis using 15660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 15660-1-AP

    FC experiment of SH-SY5Y using 15660-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug CADM3 antibody (15660-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inmouse brain tissue, Jurkat cells, SH-SY5Y cells, mouse cerebellum tissue, rat brain tissue
Positive FC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15660-1-AP targets CADM3 in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen CADM3 fusion protein Ag8081 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell adhesion molecule 3
Synonyms BIgR, Brain immunoglobulin receptor, CADM3, cell adhesion molecule 3, IGSF4B, Necl 1, NECL1, Nectin like protein 1, synCAM3, TSLC1 like protein 1, TSLL1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 38-50 kDa
GenBank Accession NumberBC033819
Gene Symbol CADM3
Gene ID (NCBI) 57863
RRIDAB_2068044
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CADM3, also known as IGSF4B or TSLL1, is a transmembrane protein that is involved in the cell-cell adhesion. CADM3 is mainly expressed in the nervous system and plays a role in the formation of synapses, axon bundles, myelinated axons and cerebellar morphogenesis (PMID: 18420026).

实验方案

Product Specific Protocols
WB protocol for CADM3 antibody 15660-1-APDownload protocol
FC protocol for CADM3 antibody 15660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols