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验证数据展示

  • WB analysis using 17773-1-AP (same clone as 17773-1-PBS)

    WB analysis using 17773-1-AP (same clone as 17773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17773-1-AP (CADM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17773-1-PBS in a different storage buffer formulation.

  • WB analysis using 17773-1-AP (same clone as 17773-1-PBS)

    WB analysis using 17773-1-AP (same clone as 17773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17773-1-AP (CADM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17773-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 17773-1-AP (same clone as 17773-1-PBS)

    IHC staining of mouse brain using 17773-1-AP (same clone as 17773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17773-1-AP (CADM2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17773-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 17773-1-AP (same clone as 17773-1-PBS)

    IHC staining of mouse brain using 17773-1-AP (same clone as 17773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17773-1-AP (CADM2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17773-1-PBS in a different storage buffer formulation.

CADM2 Polyclonal antibody, PBS Only

CADM2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17773-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Cell adhesion molecule 2, IgSF4D, Necl 3, NECL3, NECL-3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17773-1-PBS targets CADM2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12191

Product name: Recombinant human CADM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-337 aa of BC105999

Sequence: VKGSQGQFPLTQNVTVVEGGTAILTCRVDQNDNTSLQWSNPAQQTLYFDDKKALRDNRIELVRASWHELSISVSDVSLSDEGQYTCSLFTMPVKTSKAYLTVLGVPEKPQISGFSSPVMEGDLMQLTCKTSGSKPAADIRWFKNDKEIKDVKYLKEEDANRKTFTVSSTLDFRVDRSDDGVAVICRVDHESLNATPQVAMQVLEIHYTPSVKIIPSTPFPQEGQPLILTCESKGKPLPEPVLWTKDGGELPDPDRMVVSGRELNILFLNKTDNGTYRCEATNTIGQSSAEYVLIVHDPNALAGQNGPDHA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cell adhesion molecule 2
别名 Cell adhesion molecule 2, IgSF4D, Necl 3, NECL3, NECL-3
计算分子量 404 aa, 44 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC105999
基因名称 CADM2
Gene ID (NCBI) 253559
RRIDAB_3741931
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N3J6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CADM2, also known as IGSF4D, NECL3, SYNCAM2, is a member of the synaptic cell adhesion molecule 1 (SynCAM) family which belongs to the immunoglobulin (Ig) superfamily. CADM2 is a conserved modular organization comprising three Ig domains, a single transmembrane pass and a short cytoplasmic region containing 4.1 and PDZ binding motifs. CADM2 is a plasma membrane protein that accumulates in several tissues, including those of the central and peripheral nervous system, where it localizes to myelinated axons and in ependymal cells (PMID: 16311015, 17967169). The molecular weight of the CADM2 protein is approximately 50-60 kDa, which is due to N-linked glycosylation (PMID: 17967169, 21456004).

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