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CACNB4 Polyclonal antibody

CACNB4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17770-1-AP

Print datasheet

Synonyms

CAB4, CACNB4, CACNLB4, EA5, EJM

验证数据展示

  • WB analysis using 17770-1-AP

    WB analysis using 17770-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17770-1-AP (CACNB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 17770-1-AP

    IHC staining of mouse brain using 17770-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17770-1-AP (CACNB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17770-1-AP

    IHC staining of mouse brain using 17770-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17770-1-AP (CACNB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 17770-1-AP is CACNB4 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CACNB4 fusion protein Ag12178 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium channel, voltage-dependent, beta 4 subunit
Synonyms CAB4, CACNB4, CACNLB4, EA5, EJM
Calculated Molecular Weight 520 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC075049
Gene Symbol CACNB4
Gene ID (NCBI) 785
RRIDAB_2878438
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00305
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CACNB4 antibody 17770-1-APDownload protocol
IHC protocol for CACNB4 antibody 17770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Ther Nucleic Acids

circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p.

Authors - Cui Ma
View Article