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验证数据展示

  • WB analysis using 26674-1-AP

    WB analysis using 26674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26674-1-AP (CACNA2D4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 26674-1-AP

    IHC staining of mouse brain using 26674-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26674-1-AP (CACNA2D4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 26674-1-AP

    IHC staining of mouse brain using 26674-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26674-1-AP (CACNA2D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CACNA2D4 Polyclonal antibody

CACNA2D4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26674-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

RCD4, Voltage-dependent calcium channel subunit alpha-2/delta-4, Voltage-dependent calcium channel subunit alpha-2-4, Voltage-dependent calcium channel subunit delta-4, Voltage-gated calcium channel subunit alpha-2/delta-4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26674-1-AP targets CACNA2D4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CACNA2D4 fusion protein Ag24464 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium channel, voltage-dependent, alpha 2/delta subunit 4
别名 RCD4, Voltage-dependent calcium channel subunit alpha-2/delta-4, Voltage-dependent calcium channel subunit alpha-2-4, Voltage-dependent calcium channel subunit delta-4, Voltage-gated calcium channel subunit alpha-2/delta-4
计算分子量 1137 aa, 128 kDa
观测分子量100 kDa
GenBank蛋白编号BC150186
基因名称 CACNA2D4
Gene ID (NCBI) 93589
RRIDAB_3669562
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z3S7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calcium voltage-gated channel auxiliary subunit alpha2delta 4 (CACNA2D4, also known as RCD4) is a member of the alpha-2/delta subunit family within the voltage-dependent calcium channel complex. The α2δ4 subunit of retinal voltage-gated calcium channels (Cav), is crucial for developing and mature exocytotic function of the photoreceptor ribbon synapse (PMID: 29875267). CACNA2D4 is localized to the retina, particularly in photoreceptors, where it is essential for organizing synaptic ribbons and setting Cav1.4 voltage sensitivity (PMID: 28262416). In humans, mutations in CACNA2D4 are associated with autosomal recessive cone dystrophy, a condition that affects the photoreceptor cells in the retina (PMID: 16877424).

实验方案

Product Specific Protocols
WB protocol for CACNA2D4 antibody 26674-1-APDownload protocol
IHC protocol for CACNA2D4 antibody 26674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols