验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, pig brain tissue, rat brain tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27453-1-AP targets CACNA2D1 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, pig, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CACNA2D1 fusion protein Ag26190 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calcium channel, voltage-dependent, alpha 2/delta subunit 1 |
| 别名 | CACNA2, CACNA2D1, CACNL2A, CCHL2A, MHS3 |
| 计算分子量 | 125 kDa |
| 观测分子量 | 125 kDa |
| GenBank蛋白编号 | NM_000722 |
| 基因名称 | CACNA2D1 |
| Gene ID (NCBI) | 781 |
| RRID | AB_2880874 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P54289 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CACNA2D1 antibody 27453-1-AP | Download protocol |
| IHC protocol for CACNA2D1 antibody 27453-1-AP | Download protocol |
| IF protocol for CACNA2D1 antibody 27453-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Behav Immun Microglial IL-10 and β-endorphin expression mediates gabapentinoids antineuropathic pain. | ||
Heliyon Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1 | ||
iScience Exercise training affects calcium ion transport by downregulating the CACNA2D1 protein to reduce hypertension-induced myocardial injury in mice | ||
J Gastroenterol CACNA2D1 regulates the progression and influences the microenvironment of colon cancer
| ||
Neurochem Res Scavenger Receptor Class B Type I Modulates Epileptic Seizures and Receptor α2δ-1 Expression | ||
J Proteome Res ChIP-seq and RNA-seq Reveal the Involvement of Histone Lactylation Modification in Gestational Diabetes Mellitus |
