验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
27225-1-AP targets CACNA1E in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag25944 Product name: Recombinant human CACNA1E protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 801-900 aa of NM_000721 Sequence: MEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calcium channel, voltage-dependent, R type, alpha 1E subunit |
| 别名 | CACH6, CACNL1A6, Cav2.3 |
| 计算分子量 | 262 kDa |
| 观测分子量 | 240 kDa |
| GenBank蛋白编号 | NM_000721 |
| 基因名称 | CACNA1E |
| Gene ID (NCBI) | 777 |
| RRID | AB_2880808 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15878 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CACNA1E is a gene encoding the ion-conducting α1 subunit of R-type voltage-dependent calcium channels, genetic variability of CACNA1E is associated to risk of type 2 diabetes, insulin resistance and impaired insulin secretion in nondiabetic subjects.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CACNA1E antibody 27225-1-AP | Download protocol |
| WB protocol for CACNA1E antibody 27225-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Alzheimer's disease patient-derived high-molecular-weight tau impairs bursting in hippocampal neurons | ||
Elife β2-subunit alternative splicing stabilizes Cav2.3 Ca2+ channel activity during continuous midbrain dopamine neuron-like activity. |
