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验证数据展示

  • WB analysis using 30625-1-AP

    WB analysis using 30625-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30625-1-AP (CACNA1B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 30625-1-AP

    IF Staining of mouse cerebellum using 30625-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using CACNA1B antibody (30625-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CACNA1B Polyclonal antibody

CACNA1B Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 30625-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

BIII, CACH5, CACNL1A5, CACNN, Calcium channel, L type, alpha-1 polypeptide isoform 5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, Y79 cells
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30625-1-AP targets CACNA1B in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag33516

Product name: Recombinant human CACNA1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1841-1995 aa of NM_000718

Sequence: VPPHKPDEMTVGKVYAALMIFDFYKQNKTTRDQMQQAPGGLSQMGPVSLFHPLKATLEQTQPAVLRGARVFLRQKSSTSLSNGGAIQNQESGIKESVSWGTQRTQDAPHEARPPLERGHSTEIPVGRSGALAVDVQMQSITRRGPDGEPQPGLES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium channel, voltage-dependent, N type, alpha 1B subunit
别名 BIII, CACH5, CACNL1A5, CACNN, Calcium channel, L type, alpha-1 polypeptide isoform 5
计算分子量262 kDa
观测分子量200 kDa
GenBank蛋白编号NM_000718
基因名称 CACNA1B
Gene ID (NCBI) 774
RRIDAB_3669741
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ00975
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CACNA1B, also named CACH5, CACNL1A5, and BIII, belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division, and cell death. CACNA1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). CACNA1B may play a role in the directed migration of immature neurons.

实验方案

Product Specific Protocols
WB protocol for CACNA1B antibody 30625-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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