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验证数据展示

  • WB analysis using 83350-4-RR (same clone as 83350-4-PBS)

    WB analysis using 83350-4-RR (same clone as 83350-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83350-4-RR (CACHD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83350-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83350-4-PBS

    Affinity and Kinetic Characterization of 83350-4-PBS

    Biolayer interferometry (BLl) kinetic assays of 83350-4-RR against Human CACHD1 were performed. The affinity constant is 6.83 nM.

CACHD1 Recombinant monoclonal antibody, PBS Only

CACHD1 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83350-4-PBS

产品说明书

CloneNo. 240197A2

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

VWCD1, RP4 655E10.1, KIAA1573, Cache domain-containing protein 1, cache domain containing 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83350-4-PBS targets CACHD1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag22138

Product name: Recombinant human CACHD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-348 aa of BC039301

Sequence: MSTFVSSVKSSDSPTQHAVGFQKAFQLIRSTNNNTKFQANTDMVIIYLSAGITSKDSSEEDKKATLQVINEENSFLNNSVMILTYALMNDGVTGLKELAFLRDLAEQNSGKYGVPDRTALPVIKGSMMVLNQLSNLETTVGRFYTNLPNRMIDEAVFSLPFSDEMGDGLIMTVSKPCYFGNLLLGIVGVDVNLAYILEDVTYYQDSLASYTFLIDDKGYTLMHPSLTRPYLLSEPPLHTDIIHYENIPKFELVRQNILSLPLGSQIIAVPVNSSLSWHINKLRETGKEAYNVSYAWKMVQDTSFILCIVVIQPEIPVKQLKNLNTVPSSKLLYHRLDLLGQPSACLHF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cache domain containing 1
别名 VWCD1, RP4 655E10.1, KIAA1573, Cache domain-containing protein 1, cache domain containing 1
计算分子量 1274 aa, 142 kDa
GenBank蛋白编号BC039301
基因名称 CACHD1
Gene ID (NCBI) 57685
RRIDAB_3671008
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ5VU97
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CACHD1 (Cache domain-containing protein 1), also called VWCD1 (VWFA and cache domain-containing protein 1), is a type I membrane protein involved that modulates the activities of voltage-gated Ca(2+) channels (VGCCs) (PMID: 30181139; 30404013). CACHD1 has two isoforms, with molecular weights of 142 kDa and 109 kDa respectively. It has certain glycosylation modifications, and the molecular weight of isoform1 after glycosylation modification is 170 kDa (PMID: 30983497; 30404013). This antibody recognizes 142-170 kDa.

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