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验证数据展示

  • WB analysis using 87074-1-RR

    WB analysis using 87074-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87074-1-RR (CABYR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 87074-1-RR

    IHC staining of mouse testis using 87074-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 87074-1-RR (CABYR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 87074-1-RR

    IHC staining of mouse testis using 87074-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 87074-1-RR (CABYR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CABYR Recombinant monoclonal antibody

CABYR Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 87074-1-RR

产品说明书

CloneNo. 252259B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Calcium binding protein 86, Calcium-binding protein 86, Calcium-binding tyrosine phosphorylation-regulated protein, Cancer/testis antigen 88, CBP86

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue, human testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87074-1-RR targets CABYR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3012

Product name: Recombinant human CABYR protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 3-379 aa of BC011996

Sequence: SSKPRLVVPYGLKTLLEGISRAVLKTNPSNINQFAAAYFQELTMYRGNTTMDIKDLVKQFHQIKVEKWSEGTTPQKKLECLKEPGKTSVESKVPTQMEKSTDTDEDNVTRTEYSDKTTQFPSVYAVPGTEQTEAVGGLSSKPATPKTTTPPSSPPPTAVSPEFAYVPADPAQLAAQMLAMATSERGQPPPCSNMWTLYCLTDKNQQGHPSPPPAPGPFPQATLYLPNPKDPQFQQHPPKVTFPTYVMGDTKKTSAPPFILVGSNVQEAQGWKPLPGHAVVSQSDVLRYVAMQVPIAVPADEKYQKHTLSPQNANPPSGQDVPRPKSPVFLSVAFPVEDVAKKSSGSGDKCAPFGSYGIAGEVTVTTAHKRRKAETEN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium binding tyrosine-(Y)-phosphorylation regulated
别名 Calcium binding protein 86, Calcium-binding protein 86, Calcium-binding tyrosine phosphorylation-regulated protein, Cancer/testis antigen 88, CBP86
计算分子量 53 kDa
观测分子量53 kDa
GenBank蛋白编号BC011996
基因名称 CABYR
Gene ID (NCBI) 26256
RRIDAB_3745319
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75952
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CABYR antibody 87074-1-RRDownload protocol
WB protocol for CABYR antibody 87074-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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