验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, mouse testis tissue |
| Positive IHC detected in | human testis tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12351-1-AP targets CABYR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3012 Product name: Recombinant human CABYR protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 3-379 aa of BC011996 Sequence: SSKPRLVVPYGLKTLLEGISRAVLKTNPSNINQFAAAYFQELTMYRGNTTMDIKDLVKQFHQIKVEKWSEGTTPQKKLECLKEPGKTSVESKVPTQMEKSTDTDEDNVTRTEYSDKTTQFPSVYAVPGTEQTEAVGGLSSKPATPKTTTPPSSPPPTAVSPEFAYVPADPAQLAAQMLAMATSERGQPPPCSNMWTLYCLTDKNQQGHPSPPPAPGPFPQATLYLPNPKDPQFQQHPPKVTFPTYVMGDTKKTSAPPFILVGSNVQEAQGWKPLPGHAVVSQSDVLRYVAMQVPIAVPADEKYQKHTLSPQNANPPSGQDVPRPKSPVFLSVAFPVEDVAKKSSGSGDKCAPFGSYGIAGEVTVTTAHKRRKAETEN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calcium binding tyrosine-(Y)-phosphorylation regulated |
| 别名 | CABYR, Calcium binding protein 86, Cancer/testis antigen 88, CBP86, CT88, Fibrousheathin 2, Fibrousheathin II, FSP 2, FSP2 |
| 计算分子量 | 53 kDa |
| 观测分子量 | 53 kDa, 86-90 kDa |
| GenBank蛋白编号 | BC011996 |
| 基因名称 | CABYR |
| Gene ID (NCBI) | 26256 |
| RRID | AB_2069257 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75952 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CABYR antibody 12351-1-AP | Download protocol |
| WB protocol for CABYR antibody 12351-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Genet Genomics Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice | ||
J Cell Sci CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa.
| ||
J Cell Sci Radial spoke head 6 homolog A is required for sperm flagellum formation and male fertility in mice. | ||
Nat Commun Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella. | ||
