验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, A549 cells, MDA-MB-231 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26213-1-AP targets CAAP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag23850 Product name: Recombinant human C9orf82 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-361 aa of BC014658 Sequence: MTGKKSSREKRRKRSSQEAAAALAAPDIVPALASGSSGSTSGCGSAGGCGSVSCCGNANFSGSVTGGGSGGSCWGGSSVERSERRKRRSTDSSSVSGSLQQETKYILPTLEKELFLAEHSDLEEGGLDLTVSLKPVSFYISDKKEMLQQCFCIIGEKKLQKMLPDVLKNCSIEEIKKLCQEQLELLSKKKILKILEGDNGMDSDMEEEADDGSKMGSDLVSQQDICIDSASSVRENKQPEGLELKQGKGEDSDVLSINADAYDSDIEGPCNEEAAAPEAPENTVQSEAGQIDDLEKDIEKSVNEILGLAESSPNEPKAATLAVPPPEDVQPSAQQLELLELEMRARAIKALMKAGDIKKPA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 9 open reading frame 82 |
| 别名 | C9orf82, CAAP, Caspase activity and apoptosis inhibitor 1, Conserved anti-apoptotic protein, RP11 337A23.1 |
| 观测分子量 | 47-55 kDa |
| GenBank蛋白编号 | BC014658 |
| 基因名称 | C9orf82 |
| Gene ID (NCBI) | 79886 |
| RRID | AB_3742173 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H8G2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAAP1 (Caspase Activity and Apoptosis Inhibitor 1), also known as C9orf82, is an evolutionarily conserved anti-apoptotic protein. It inhibits apoptosis by interfering with the activation of caspase-10, thereby blocking the feedback amplification loop of caspase-3/9 in the mitochondrial apoptotic pathway. Recent studies have revealed that CAAP1 plays a dual role in the regulation of autophagy, balancing the processes of autophagy and apoptosis. In tumor biology, the expression level of CAAP1 is associated with chemotherapy resistance in various cancers, making it a potential prognostic biomarker. In addition, CAAP1 is involved in neuroprotective mechanisms and plays an important role in hypoxic-ischemic brain injury by regulating neuronal apoptosis. Its expression is finely regulated by non-coding RNA networks, including MKL1/miR-5100 and miR-184-5p. (PMID: 30629682)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CAAP1 antibody 26213-1-AP | Download protocol |
| IP protocol for CAAP1 antibody 26213-1-AP | Download protocol |
| WB protocol for CAAP1 antibody 26213-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
