验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22241-1-AP targets CA5A in IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag17689 Product name: Recombinant human CA5A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 77-126 aa of BC137411 Sequence: DPQLKPLRVSYEAASCLYIWNTGYLFQVEFDDATEASGISGGPLENHYRL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | carbonic anhydrase VA, mitochondrial |
| 别名 | CA VA, CA5, Carbonate dehydratase VA, Carbonic anhydrase 5A, mitochondrial, Carbonic anhydrase VA |
| 计算分子量 | 305 aa, 35 kDa |
| GenBank蛋白编号 | BC137411 |
| 基因名称 | CA5A |
| Gene ID (NCBI) | 763 |
| RRID | AB_3742068 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35218 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Carbonic anhydrase VA (CA-VA) is a mitochondrial enzyme that is highly expressed in the liver and functions to provide bicarbonate as a substrate for other enzymatic reactions.CA-VA deficiency , which is caused by biallelic pathogenic variants in the CA5A gene, is characterized by neonatal metabolic hyperammonemic encephalopathy, lactic acidosis, hypoglycemia, and ketonuria. (PMID: 41113665, PMID: 24530203)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CA5A antibody 22241-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
