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验证数据展示

  • WB analysis using 85706-1-RR

    WB analysis using 85706-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85706-1-RR (Carbonic Anhydrase 4/CA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 85706-1-RR

    IHC staining of mouse lung using 85706-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85706-1-RR (Carbonic Anhydrase 4/CA4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85706-1-RR

    Affinity and Kinetic Characterization of 85706-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85706-1-RR against Human CA4 were performed. The affinity constant is 9.62 nM.

Carbonic Anhydrase 4/CA4 Recombinant antibody

Carbonic Anhydrase 4/CA4 Recombinant Antibody for WB, IHC, ELISA
Cat No. 85706-1-RR

产品说明书

CloneNo. 242975A3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CA4, CA IV, CAIV, CA-IV, Car4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85706-1-RR targets Carbonic Anhydrase 4/CA4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Carbonic Anhydrase 4/CA4 fusion protein Ag4960 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carbonic anhydrase IV
别名 CA4, CA IV, CAIV, CA-IV, Car4
计算分子量 35 kDa
观测分子量35 kDa
GenBank蛋白编号BC057792
基因名称 CA4
Gene ID (NCBI) 762
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP22748
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA4(Carbonic anhydrase 4) is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary, chorionic (and certain other) capillaries and of proximal renal tubules. It plays a critical role by maintaining the pH in the outer retina, which is important for the normal function of photoreceptors. This protein belongs to the alpha-carbonic anhydrase family.

实验方案

Product Specific Protocols
WB protocol for Carbonic Anhydrase 4/CA4 antibody 85706-1-RRDownload protocol
IHC protocol for Carbonic Anhydrase 4/CA4 antibody 85706-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols