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验证数据展示

  • WB analysis using 16961-1-AP (same clone as 16961-1-PBS)

    WB analysis using 16961-1-AP (same clone as 16961-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 16961-1-AP (same clone as 16961-1-PBS)

    IP experiment of mouse kidney using 16961-1-AP (same clone as 16961-1-PBS)

    IP result of anti-CA2 (IP:16961-1-AP, 4ug; Detection:16961-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

CA2 Polyclonal antibody, PBS Only

CA2 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 16961-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, Indirect ELISA

别名

Carbonate dehydratase II, Car2, CA-II, CAII, CAC

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16961-1-PBS targets CA2 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag10680

Product name: Recombinant human CA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC011949

Sequence: MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFAARGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbonic anhydrase II
别名 Carbonate dehydratase II, Car2, CA-II, CAII, CAC
计算分子量 260 aa, 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC011949
基因名称 CA2
Gene ID (NCBI) 760
RRIDAB_2065857
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00918
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CA2(carbonic anhydrase II ) is also called Car2, CA-II, and CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in brain development (PMID:16825953). Defects in CA2 cause osteopetrosis autosomal recessive type 3 (OPTB3).

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