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验证数据展示

  • WB analysis using 85595-3-RR (same clone as 85595-3-PBS)

    WB analysis using 85595-3-RR (same clone as 85595-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85595-3-RR (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85595-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01993-1

    Sandwich ELISA standard curve of MP01993-1

    Sandwich ELISA standard curve of MP01993-1, Human CA12 Recombinant Matched Antibody Pair - PBS only. 85595-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7107. 85595-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • Sample test of MP01993-1

    Sample test of MP01993-1

    The mean CA12 concentration was determined to be 93.99 ng/mL in A549 cell extract based on a 3.10 mg/mL extract load.

  • Cytometric bead array standard curve of MP01993-1

    Cytometric bead array standard curve of MP01993-1

    Cytometric bead array standard curve of MP01993-1, CA12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85595-3-PBS. Detection antibody: 85595-2-PBS. Standard: Ag7107. Range: 0.781-100 ng/mL.

CA12 Recombinant antibody, PBS Only (Capture)

CA12 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85595-3-PBS

产品说明书

CloneNo. 243053B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

CA XII, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CA12 Ready-To-Use IHC Kit. CA12 primary antibody included.

产品信息

85595-3-PBS targets CA12 as part of a matched antibody pair:

MP01993-1: 85595-3-PBS capture and 85595-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 CA12 fusion protein Ag7107 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carbonic anhydrase XII
别名 CA XII, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII
计算分子量 39 kDa
观测分子量38-45 kDa
GenBank蛋白编号BC000278
基因名称 CA12
Gene ID (NCBI) 771
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43570
储存缓冲液 PBS only{{ptg:BufferTemp}}7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CA12 belongs to the alpha-carbonic anhydrase family. It reverses the hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.