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验证数据展示

  • WB analysis using 85357-1-RR

    WB analysis using 85357-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85357-1-RR (C9orf89 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 85357-1-RR

    IHC staining of mouse kidney using 85357-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85357-1-RR (C9orf89 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 85357-1-RR

    IHC staining of mouse kidney using 85357-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85357-1-RR (C9orf89 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 85357-1-RR

    IF Staining of A431 using 85357-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C9orf89 antibody (85357-1-RR, Clone: 242638F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85357-1-RR

    Affinity and Kinetic Characterization of 85357-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85357-1-RR against Human C9orf89 were performed. The affinity constant is 0.756 nM.

C9orf89 Recombinant antibody

C9orf89 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85357-1-RR

产品说明书

CloneNo. 242638F7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

242638F7, Bcl10-interacting CARD protein, BinCARD, CARD19, Caspase recruitment domain-containing protein 19

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, C2C12 cells, H9C2 cells, rat kidney tissue, mouse kidney tissue, human placenta tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85357-1-RR targets C9orf89 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 C9orf89 fusion protein Ag14084 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 9 open reading frame 89
别名 242638F7, Bcl10-interacting CARD protein, BinCARD, CARD19, Caspase recruitment domain-containing protein 19
计算分子量 228 aa, 26 kDa
观测分子量15-21 kDa
GenBank蛋白编号BC004500
基因名称 C9orf89
Gene ID (NCBI) 84270
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96LW7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C9orf89 (also known as CARD19 or BinCARD) is a mitochondria-localized protein that inhibits BCL10-induced NF-κB activation mainly through its caspase recruitment structural domain (CARD). It plays a role in T cell receptor (TCR) signaling, but deletion of endogenous CARD19 has a lesser effect on Bcl10-dependent NF-κB activation. In addition, C9orf89 is expressed in multiple cell types and is involved in the regulation of inflammatory and immune responses.

实验方案

Product Specific Protocols
WB protocol for C9orf89 antibody 85357-1-RRDownload protocol
IHC protocol for C9orf89 antibody 85357-1-RRDownload protocol
IF protocol for C9orf89 antibody 85357-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols