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验证数据展示

  • WB analysis of MDA-MB-453s using 20848-1-AP

    WB analysis of MDA-MB-453s using 20848-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20848-1-AP (C9orf86 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 20848-1-AP

    IHC staining of human breast cancer using 20848-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20848-1-AP (C9orf86 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C9orf86 antibody included
  • IHC staining of human breast cancer using 20848-1-AP

    IHC staining of human breast cancer using 20848-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20848-1-AP (C9orf86 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C9orf86 antibody included
  • IF Staining of HeLa using 20848-1-AP

    IF Staining of HeLa using 20848-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20848-1-AP (C9orf86 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 20848-1-AP

    IF Staining of HeLa using 20848-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C9orf86 antibody (20848-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C9orf86 Polyclonal antibody

C9orf86 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20848-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

RBEL1, Rab-like protein 6, Rab-like protein 1, pp8875, Partner of ARF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy C9orf86 Ready-To-Use IHC Kit. C9orf86 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20848-1-AP targets C9orf86 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 C9orf86 fusion protein Ag14350 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 86
别名 RBEL1, Rab-like protein 6, Rab-like protein 1, pp8875, Partner of ARF
计算分子量 729 aa, 80 kDa
观测分子量 125 kDa
GenBank蛋白编号BC002945
基因名称 C9orf86
Gene ID (NCBI) 55684
RRIDAB_2878750
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3YEC7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C9orf86, also known as RBEL1 (Rab-like protein 1), is a novel subfamily of GTPases within the Ras superfamily. It has two splice variants, RBEL1Aand RBEL1B. Unlike known Rabs that aremostly cytosolic, RBEL1B predominantly resides in the nucleus, whereas RBEL1A is localized primarily to the cytosol. C9orf86 is overexpressed in the majority of primary breast tumors, and knockdown of C9orf86 in MCF-7 breast cancer cells resulted in cell growth suppression associated with apoptosis [PMID: 23977139]. Western blot analysis revealed that the endogenous RBEL1 migrated at 110-125 kDa, 80 kDa, maybe due to a number of potential O-linked glycosylation sites.[PMID: 17962191]

实验方案

Product Specific Protocols
WB protocol for C9orf86 antibody 20848-1-APDownload protocol
IHC protocol for C9orf86 antibody 20848-1-APDownload protocol
IF protocol for C9orf86 antibody 20848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

m5C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization

Authors - Ning Wang
View Article