验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21330-1-AP targets C9orf7 / CACFD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | C9orf7 / CACFD1 fusion protein Ag15336 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 9 open reading frame 7 |
| 别名 | C9orf7, CACFD1, Calcium channel flower homolog, D9S2135, FWE, hFWE, PSEC0248, UNQ3071/PRO9903 |
| 计算分子量 | 172 aa, 18 kDa |
| 观测分子量 | ~25 kDa |
| GenBank蛋白编号 | BC030558 |
| 基因名称 | CACFD1 |
| Gene ID (NCBI) | 11094 |
| RRID | AB_3085651 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UGQ2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calcium Channel Flower Domain Containing 1 (CACFD1) also named as C9orf7 or human Flower protein (hFWE). In Drosophila, cell selection uses a cell selection mechanism based on 'fitness fingerprints', which allow it to delay ageing, prevent developmental malformations and replace old tissues during regeneration. At the molecular level, these fitness fingerprints consist of combinations of Flower membrane proteins. Proteins that indicate reduced fitness are called Flower-Lose, because they are expressed in cells marked to be eliminated. It has different isoforms, the calculated MW rang from 14-25 kDa. 21330-1-AP can detect a band around 25 kDa.(PMID:31341286)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C9orf7 / CACFD1 antibody 21330-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |