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验证数据展示

  • WB analysis of mouse skeletal muscle using 25630-1-AP

    WB analysis of mouse skeletal muscle using 25630-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25630-1-AP (C9orf61 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 25630-1-AP

    IHC staining of human heart using 25630-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 25630-1-AP

    IHC staining of human heart using 25630-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 40x lens).

C9orf61 Polyclonal antibody

C9orf61 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25630-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

Endosomal transmembrane binding with epsin, Endosomal transmembrane epsin interactor 1, ENTREP1, FAM189A2, Protein FAM189A2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25630-1-AP targets C9orf61 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, CoIP
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag22242

Product name: Recombinant human C9orf61 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 288-450 aa of BC113685

Sequence: PVLSCEAATQTERRLDLAAVTLRRGLRSRASRCRPRSLIDYKSYMDTKLLVARFLEQSSCTMTPDIHELVENIKSVLKSDEEHMEEAITSASFLEQIMAPLQPSTSRAHKLPSRRQPGLLHLQSCGDLHTFTPAGRPRAERRPRRVEAERPHSLIGVIRETVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 61
别名 Endosomal transmembrane binding with epsin, Endosomal transmembrane epsin interactor 1, ENTREP1, FAM189A2, Protein FAM189A2
计算分子量 450 aa, 50 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC113685
基因名称 C9orf61
Gene ID (NCBI) 9413
RRIDAB_2880170
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15884
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C9orf61, also named as FAM189A2 and X123, is promoinently expressed in muscle. It has three isoforms with MW 45-50 kDa, 32-35 kDa and 27 kDa.

实验方案

Product Specific Protocols
WB protocol for C9orf61 antibody 25630-1-APDownload protocol
IHC protocol for C9orf61 antibody 25630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,CoIP

Tissue Cell

FAM189A2 plays a tumour suppressor role in lung adenocarcinoma by influencing cell apoptosis, CXCR4 expression and tight junction proteins

Authors - Jiakun Liu
View Article