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C9orf61 Polyclonal antibody

C9orf61 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25630-1-AP

Print datasheet

Synonyms

C9orf61, FAM189A2, Protein FAM189A2, Protein X123, RP11 548B3.1, X123

验证数据展示

  • WB analysis of mouse skeletal muscle using 25630-1-AP

    WB analysis of mouse skeletal muscle using 25630-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25630-1-AP (C9orf61 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 25630-1-AP

    IHC staining of human heart using 25630-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 25630-1-AP

    IHC staining of human heart using 25630-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25630-1-AP is C9orf61 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen C9orf61 fusion protein Ag22242 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 9 open reading frame 61
Synonyms C9orf61, FAM189A2, Protein FAM189A2, Protein X123, RP11 548B3.1, X123
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC113685
Gene Symbol C9orf61
Gene ID (NCBI) 9413
RRIDAB_2880170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15884
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C9orf61, also named as FAM189A2 and X123, is promoinently expressed in muscle. It has three isoforms with MW 45-50 kDa, 32-35 kDa and 27 kDa.

实验方案

Product Specific Protocols
WB protocol for C9orf61 antibody 25630-1-APDownload protocol
IHC protocol for C9orf61 antibody 25630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols