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验证数据展示

  • WB analysis of mouse skeletal muscle using 25630-1-AP

    WB analysis of mouse skeletal muscle using 25630-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25630-1-AP (C9orf61 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 25630-1-AP

    IHC staining of human heart using 25630-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 25630-1-AP

    IHC staining of human heart using 25630-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 40x lens).

C9orf61 Polyclonal antibody

C9orf61 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25630-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

C9orf61, FAM189A2, Protein FAM189A2, Protein X123, RP11 548B3.1, X123

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25630-1-AP targets C9orf61 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, CoIP
经测试反应性 human, mouse
文献引用反应性human
免疫原 C9orf61 fusion protein Ag22242 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 61
别名 C9orf61, FAM189A2, Protein FAM189A2, Protein X123, RP11 548B3.1, X123
计算分子量 450 aa, 50 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC113685
基因名称 C9orf61
Gene ID (NCBI) 9413
RRIDAB_2880170
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15884
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C9orf61, also named as FAM189A2 and X123, is promoinently expressed in muscle. It has three isoforms with MW 45-50 kDa, 32-35 kDa and 27 kDa.

实验方案

Product Specific Protocols
WB protocol for C9orf61 antibody 25630-1-APDownload protocol
IHC protocol for C9orf61 antibody 25630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,CoIP

Tissue Cell

FAM189A2 plays a tumour suppressor role in lung adenocarcinoma by influencing cell apoptosis, CXCR4 expression and tight junction proteins

Authors - Jiakun Liu
View Article