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验证数据展示

  • WB analysis of HepG2 using 24068-1-AP

    WB analysis of HepG2 using 24068-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24068-1-AP

    IF Staining of HepG2 using 24068-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C9orf41/CARNMT1 antibody (24068-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

C9orf41/CARNMT1 Polyclonal antibody

C9orf41/CARNMT1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24068-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, ELISA

别名

C9orf41, CARNMT1, Carnosine N-methyltransferase, EC:2.1.1.22

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24068-1-AP targets C9orf41/CARNMT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原

CatNo: Ag21268

Product name: Recombinant human C9orf41 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 61-409 aa of BC034033

Sequence: LEREHFWKIINAFRYYGTSMHERVNRTERQFRSLPANQQKLLPQFLLHLDKIRKCIDHNQEILLTIVNDCIHMFENKEYGEDGNGKIMPASTFDMDKLKSTLKQFVRDWSETGKAERDACYQPIIKEILKNFPKERWDPSKVNILVPGAGLGRLAWEIAMLGYACQGNEWSFFMLFSSNFVLNRCSEINKYKLYPWIHQFSNNRRSADQIRPIFFPDVDPHSLPPGSNFSMTAGDFQEIYSECNTWDCIATCFFIDTAHNVIDYIDTIWKILKPGGIWINLGPLLYHFENLANELSIELSYEDIKNVVLQYGFKVEVEKESVLSTYTVNDLSMMKYYYECVLFVVRKPQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 41
别名 C9orf41, CARNMT1, Carnosine N-methyltransferase, EC:2.1.1.22
计算分子量 409 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC034033
基因名称 C9orf41
Gene ID (NCBI) 138199
RRIDAB_2879425
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ8N4J0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The enzyme CARNMT1 (carnosine N-methyltransferase 1, also known as C9orf41), conserved from yeast to human, was identified as an N1 position-specific methyltransferase that catalyzes the conversion of carnosine to anserine (β-alanyl-N-π-methyl-L-histidine).

实验方案

Product Specific Protocols
IF protocol for C9orf41/CARNMT1 antibody 24068-1-APDownload protocol
WB protocol for C9orf41/CARNMT1 antibody 24068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Genes Dev

Histidine N1-position-specific methyltransferase CARNMT1 targets C3H zinc finger proteins and modulates RNA metabolism

Authors - Tadahiro Shimazu
  • KO Validated
View Article
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