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验证数据展示

  • WB analysis using 24969-1-AP

    WB analysis using 24969-1-AP

    Mouse testis tissue lysates were subjected to SDS PAGE followed by western blot with 24969-1-AP (C9orf171 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 24969-1-AP

    IF Staining of U-251 using 24969-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using C9orf171 antibody (24969-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 24969-1-AP

    IF Staining of mouse testis using 24969-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using C9orf171 antibody (24969-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

C9orf171 Polyclonal antibody

C9orf171 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA
Cat No. 24969-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IF-P, ELISA

别名

Cilia- and flagella-associated protein 77, CFAP77

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24969-1-AP targets C9orf171 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 C9orf171 fusion protein Ag21797 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 171
别名 Cilia- and flagella-associated protein 77, CFAP77
计算分子量 320 aa, 36 kDa
观测分子量33-36 kDa
GenBank蛋白编号BC118593
基因名称 C9orf171
Gene ID (NCBI) 389799
RRIDAB_3085763
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZQR2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C9orf171 antibody 24969-1-APDownload protocol
IF protocol for C9orf171 antibody 24969-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols