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验证数据展示

  • WB analysis of HeLa using 20475-1-AP

    WB analysis of HeLa using 20475-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20475-1-AP (C9orf156 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 20475-1-AP

    IHC staining of human lung cancer using 20475-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 20475-1-AP (C9orf156 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 20475-1-AP

    IHC staining of human lung cancer using 20475-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 20475-1-AP (C9orf156 antibody) at dilution of 1:50 (under 40x lens).

C9orf156 Polyclonal antibody

C9orf156 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20475-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C9orf156, HSPC219, NAP1, Nef associated protein 1, Thioesterase NAP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20475-1-AP targets C9orf156 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14335

Product name: Recombinant human C9orf156 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 92-441 aa of BC002863

Sequence: NGHLSCKAKVQPPRLNGAKTGVFSTRSPHRPNAIGLTLAKLEKVEGGAIYLSGIDMIHGTPVLDIKPYIAEYDSPQNVMEPLADFNLQNNQHTPNTVSQSDSKTDSCDQRQLSGCDEPQPHHSTKRKPKCPEDRTSEENYLTHSDTARIQQAFPMHREIAVDFGLESRRDQSSSVAEEQIGPYCPEKSFSEKGTDKKLERVEGAAVLQGSRAETQPMAPHCPAGRADGAPRSVVPAWVTEAPVATLEVRFTPHAEMDLGQLSSQDVGQASFKYFQSAEEAKRAIEAVLSADPRSVYRRKLCQDRLFYFTVDIAHVTCWFGDGFAEVLRIKPASEPVHMTGPVGSLVSLGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 156
别名 C9orf156, HSPC219, NAP1, Nef associated protein 1, Thioesterase NAP1
计算分子量 441 aa, 49 kDa
观测分子量 48-55 kDa
GenBank蛋白编号BC002863
基因名称 C9orf156
Gene ID (NCBI) 51531
RRIDAB_10665553
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BU70
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C9orf156 antibody 20475-1-APDownload protocol
IHC protocol for C9orf156 antibody 20475-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols