验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33571-1-AP targets C9orf114 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38014 Product name: Recombinant human C9orf114 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 163-340 aa of BC063644 Sequence: HQDLQFAGLLNPLDSPHHMRQDEESEFREGIVVDRPTRPGHGSFVNCGMKKEVKIDKNLEPGLRVTVRLNQQQHPDCKTYHGKVVSSQDPRTKAGLYWGYTVRLASCLSAVFAEAPFQDGYDLTIGTSERGSDVASAQLPNFRHALVVFGGLQGLEAGADADPNLEVAEPSVLFDLYV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 9 open reading frame 114 |
| 别名 | CENP-32, Centromere protein 32, EC:2.1.1.-, Kinetochore-associated protein, Putative methyltransferase C9orf114 |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC063644 |
| 基因名称 | C9orf114 |
| Gene ID (NCBI) | 51490 |
| RRID | AB_3743014 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q5T280 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPOUT1 (SPOUT domain-containing methyltransferase 1), also known as CENP32 or C9orf114, is a methyltransferase containing a SPOUT domain. This protein is highly expressed in the nucleolus and is crucial for ribosome function. SPOUT1 has been identified as an essential protein for cell survival in both Drosophila and humans. Moreover, mutations in SPOUT1 are associated with developmental epileptic encephalopathy (DEE) in humans, characterized by infantile seizures and intellectual disability.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C9orf114 antibody 33571-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
