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验证数据展示

  • WB analysis using 15587-1-AP

    WB analysis using 15587-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15587-1-AP (HIG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 15587-1-AP

    IHC staining of mouse kidney using 15587-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15587-1-AP (HIG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 15587-1-AP

    IF Staining of A549 using 15587-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIG2 antibody (15587-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

HIG2 Polyclonal antibody

HIG2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15587-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

C7orf68, HIG 2, HIG-2, HILPDA, Hypoxia-inducible gene 2 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15587-1-AP targets HIG2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7829

Product name: Recombinant human C7orf68 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-63 aa of BC001863

Sequence: MKHVLNLYLLGVVLTLLSIFVRVMESLEGLLESPSPGTSWTTRSQLANTEPTKGLPDHPSRSM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 7 open reading frame 68
别名 C7orf68, HIG 2, HIG-2, HILPDA, Hypoxia-inducible gene 2 protein
计算分子量 7 kDa
观测分子量 7-10 kDa
GenBank蛋白编号BC001863
基因名称 C7orf68
Gene ID (NCBI) 29923
RRIDAB_3669213
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5L2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIG2 (Hypoxia inducible gene 2), also known as hypoxia inducible lipid droplet associated (HILPDA), is a gene that plays a significant role in the context of hypoxia, particularly in cancer biology. HIG2 is can be induced by hypoxia and glucose deprivation. It is expressed under low-oxygen conditions, which are common in the tumor microenvironment, and has been identified as a target gene of hypoxia-inducible factor-1 (HIF-1). HIG2 is implicated in the development and progression of various types of cancer, including renal cell carcinoma(PMID: 15930302), cell lymphoma(PMID: 26757780), epithelial ovarian cancer(PMID: 20134266), and uterine cancer(PMID: 21614900). Its expression is often elevated in these cancers and is associated with poor prognosis.

实验方案

Product Specific Protocols
WB protocol for HIG2 antibody 15587-1-APDownload protocol
IHC protocol for HIG2 antibody 15587-1-APDownload protocol
IF protocol for HIG2 antibody 15587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols