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验证数据展示

  • WB analysis using 20797-1-AP

    WB analysis using 20797-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20797-1-AP (C7orf50 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of L02 using 20797-1-AP

    IF Staining of L02 using 20797-1-AP

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using 20797-1-AP (C7orf50 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach using 20797-1-AP

    IHC staining of human stomach using 20797-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 20797-1-AP (C7orf50 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach using 20797-1-AP

    IHC staining of human stomach using 20797-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 20797-1-AP (C7orf50 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C7orf50 Polyclonal antibody

C7orf50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20797-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

YCR016W, FP15621

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, Jurkat cells, L02 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

20797-1-AP targets C7orf50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 C7orf50 fusion protein Ag14709 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 7 open reading frame 50
别名 YCR016W, FP15621
计算分子量 194 aa, 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC006224
基因名称 C7orf50
Gene ID (NCBI) 84310
RRIDAB_10732839
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRJ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C7orf50 (Chromosome 7, Open Reading Frame 50) is ubiquitously expressed in the kidneys, brain, fat, prostate, spleen, among 22 other tissues and demonstrates low tissue specificity.

实验方案

Product Specific Protocols
WB protocol for C7orf50 antibody 20797-1-APDownload protocol
IHC protocol for C7orf50 antibody 20797-1-APDownload protocol
IF protocol for C7orf50 antibody 20797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Phosphorylation of the Ribosomal Protein RPL12/uL11 Affects Translation during Mitosis.

Authors - Koshi Imami
View Article
humanWB

Life Sci Alliance

The nucleolar phase of signal recognition particle assembly

Authors - Amani Issa
View Article