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验证数据展示

  • WB analysis using 27790-1-AP

    WB analysis using 27790-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27790-1-AP (C7orf43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27790-1-AP

    IHC staining of human liver using 27790-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27790-1-AP (C7orf43 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 27790-1-AP

    IHC staining of human liver using 27790-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27790-1-AP (C7orf43 antibody) at dilution of 1:200 (under 40x lens).

C7orf43 Polyclonal antibody

C7orf43 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27790-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat

应用

WB, IHC, ELISA

别名

C7orf43

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27790-1-AP targets C7orf43 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag27149

Product name: Recombinant human C7orf43 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 409-580 aa of BC015722

Sequence: EHAQAGKQLCEEERRAMQAALDSVVCHTPLNNLGFSRKGSALTFSVAFQALRTGLFELSQHMKLKLQFTASVSHPPPEARPLSRKSSPSSPAVRDLVERHQASLGRSQSFSHQQPSRSHLMRSGSVMERRAITPPVASPVGRPLYLPPDKAVLSLDKIAKRECKVLVVEPVK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 7 open reading frame 43
别名 C7orf43
观测分子量 70 kDa
GenBank蛋白编号BC015722
基因名称 C7orf43
Gene ID (NCBI) 55262
RRIDAB_2880972
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVR3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The predicted MW of C7orf43 is 62 kDa. Catalog#27790-1-AP recognises 65-70 kDa band may due to phosphorylation.

实验方案

Product Specific Protocols
WB protocol for C7orf43 antibody 27790-1-APDownload protocol
IHC protocol for C7orf43 antibody 27790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols