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验证数据展示

  • WB analysis using 27790-1-AP

    WB analysis using 27790-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27790-1-AP (C7orf43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27790-1-AP

    IHC staining of human liver using 27790-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27790-1-AP (C7orf43 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 27790-1-AP

    IHC staining of human liver using 27790-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27790-1-AP (C7orf43 antibody) at dilution of 1:200 (under 40x lens).

C7orf43 Polyclonal antibody

C7orf43 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27790-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat

应用

WB, IHC, ELISA

别名

C7orf43

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27790-1-AP targets C7orf43 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 C7orf43 fusion protein Ag27149 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 7 open reading frame 43
别名 C7orf43
观测分子量 70 kDa
GenBank蛋白编号BC015722
基因名称 C7orf43
Gene ID (NCBI) 55262
RRIDAB_2880972
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVR3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The predicted MW of C7orf43 is 62 kDa. Catalog#27790-1-AP recognises 65-70 kDa band may due to phosphorylation.

实验方案

Product Specific Protocols
WB protocol for C7orf43 antibody 27790-1-APDownload protocol
IHC protocol for C7orf43 antibody 27790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols